ENOS (Ab 1177) Antibody

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

NCBI Gene ID

4846

Swiss Prot

P29474

Accession Number

NP_000594.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

ENOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

140 kDa

Specificity

This antibody detects endogenous level of total eNOS protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

NOS3

NCBI Official Name

Nitric oxide synthase 3 (endothelial cell)

NCBI Organism

Homo sapiens

Background Reference 01

Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M. B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S. R. et al. (2002) J. Biol. Chem. 277, 6017-6024.