Histone H3.1 (Ab 10) Antibody

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

NCBI Gene ID

8351

Swiss Prot

P68431

Accession Number

NP_003521.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Histone H3.1 (Ab-10) antibody was raised against a peptide sequence around aa.8~12 (R-K-S-T-G) derived from Human Histone H3.1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

17 kDa

Specificity

This antibody detects endogenous level of total Histone H3.1 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

HIST1H3D

NCBI Official Name

Histone cluster 1, H3d

NCBI Organism

Homo sapiens

Background Reference 01

Workman, J. L. and Kingston, R. E. (1998) Annu Rev Biochem 67, 545-79. Hansen, J. C. et al. (1998) Biochemistry 37, 17637-41. Strahl, B. D. and Allis, C. D. (2000) Nature 403, 41-5. Cheung, P. et al. (2000) Cell 103, 263-71.