Chk2 (Ab 516) Antibody

Product Specifications

Background

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

NCBI Gene ID

11200

Swiss Prot

O96017

Accession Number

NP_001005735.1

Host

Rabbit

Reactivity

Human

Immunogen

Chk2 (Ab-516) antibody was raised against a peptide sequence around aa. 514~518 (Q-P-S-T-S) derived from Chk2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

62 kDa

Specificity

This antibody detects endogenous levels of total Chk2 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CHEK2

NCBI Official Name

Checkpoint kinase 2

NCBI Organism

This antibody was raised against a peptide sequence around aa. 514~518 (Q-P-S-T-S) derived from Chk2

Background Reference 01

Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. Ahn, J. Y. et al. (2000) Cancer Res. 60, 5934-5936. Lee, C. H. and Chung, J. H. (2001) J. Biol. Chem. 276, 30537-30541