Caveolin 1 (Ab 14) Antibody

Product Specifications

Background

The scaffolding protein encoded by Caveolin-1 is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

NCBI Gene ID

857

Swiss Prot

Q03135

Accession Number

NP_001166366.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Caveolin-1 (Ab-14) antibody was raised against a peptide sequence around aa.12~16 (H-L-Y-T-V) derived from Human CAVEOLIN-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

24 kDa

Specificity

This antibody detects endogenous level of total Caveolin-1 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CAV1

NCBI Official Name

Caveolin 1, caveolae protein, 22kDa

NCBI Organism

Homo sapiens

Background Reference 01

Zhang Y, et al. (2005) Mol Cell Proteomics. 4 (9) : 1240-1250. Labrecque L, et al. (2004) J Biol Chem. 279 (50) : 52132-52140. Fielding PE, et al. (2004) Biochemistry. 43 (9) : 2578-2586. Labrecque L, et al. (2003) Mol Biol Cell. 14 (1) : 334-347. Maggi D, et al. (2002) Biochem Biophys Res Commun. 295 (5) : 1085-1089.