P53 (Ab 46) Antibody

Product Specifications

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

NCBI Gene ID

7157

Swiss Prot

P04637

Accession Number

NP_000537.3

Host

Rabbit

Reactivity

Human

Immunogen

P53 (Ab-46) antibody was raised against a peptide sequence around aa. 44~48 (M-L-S-P-D) derived from Human p53.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

53 kDa

Specificity

This antibody detects endogenous level of total p53 protein.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

TP53

NCBI Official Name

Tumor protein p53

NCBI Organism

Homo sapiens

Background Reference 01

Dhavan, R. and Tsai, L. H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24 (35) : 5431-5442. Mayo LD, et al. (2005) J Biol Chem. 280 (28) : 25953-25959. Wang L, et al. (2005) Oncogene. 24 (18) : 3020-3027.