Progesterone Receptor (Ab 190) Antibody

Product Specifications

Background

Progesterone receptors (PRs) are nuclear hormone receptors of the NR3C class, which also includes mineralocorticoid, glucocorticoid and androgen receptors. They exist as homodimers coupled to Hsp90 or HMGB proteins, which are shed upon activation. The major signaling pathway used by progesterone receptors is via direct DNA binding and transcriptional regulation of target genes. They can also signal by binding to other proteins, mainly with transcription factors such as NF-κ B, AP-1 or STAT. Progesterone receptors are found in the female reproductive tract, mammary glands, brain and pituitary gland and receptor expression is induced by estrogen. Well established functions of progesterone receptors include ovulation, implantation, mammary gland development and maintenance of pregnancy. In addition, progesterone, signaling through the progesterone receptor, increases the ventilatory response of the respiratory centers to carbon dioxide and decreases arterial and alveolar PCO2 in the luteal phase of the menstrual cycle and during pregnancy. The human gene encoding the progesterone receptor has been localized to 11q22.

NCBI Gene ID

5241

Swiss Prot

P06401

Accession Number

NP_000917.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Progesterone Receptor (Ab-190) antibody was raised against a peptide sequence around aa.188~192 (G-L-S-P-A) derived from Human Progesterone Receptor.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

99 kDa

Specificity

This antibody detects endogenous level of total Progesterone Receptor protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

PGR

NCBI Official Name

Progesterone receptor

NCBI Organism

Homo sapiens

Background Reference 01

Narayanan R, et al. (2005) Mol Cell Biol; 25 (8) : 2885-98. Knotts TA, et al. (2001) J Biol Chem; 276 (11) : 8475-83. Clemm DL, et al. (2000) Mol Endocrinol; 14 (1) : 52-65. Zhang Y, et al. (1997) Mol Endocrinol; 11 (6) : 823-32