Beta catenin (phospho Tyr333) Antibody

Product Specifications

Background

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

NCBI Gene ID

1499

Swiss Prot

P35222

Accession Number

NP_001091679.1

Host

Rabbit

Reactivity

Human

Immunogen

Beta-catenin (phospho-Tyr333) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 333 (Y-T-Y (p) -E-K) derived from Human β -catenin .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

92 kDa

Specificity

This antibody detects endogenous level of β -catenin only when phosphorylated at tyrosine 333.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CTNNB1

NCBI Official Name

Catenin (cadherin-associated protein), beta 1, 88kDa

NCBI Organism

Homo sapiens

Background Reference 01

Huelsken J, et al. J. Cell Biol. 127:2061-2069 (1994) Ota T, et al. Nat. Genet. 36:40-45 (2004) The MGC Project Team. Genome Res. 14:2121-2127 (2004) Butz S, et al. FEBS Lett. 355:195-200 (1994)