CK2a (phospho Thr360 / Ser362) Antibody

Product Specifications

Background

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

NCBI Gene ID

1495

Swiss Prot

P68400

Accession Number

NP_001894.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CK2a (phospho-Thr360/Ser362) antibody was raised against a peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T (p) -P-S (p) -P-L) derived from Human CK2α .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

42 kDa

Specificity

This antibody detects endogenous level of CK2 only when phosphorylated at thronine 360.

Applications Notes

Western Blot: 1:1000

Symbol

CTNNA1

NCBI Official Name

Catenin (cadherin-associated protein), alpha 1, 102kDa

NCBI Organism

Homo sapiens

Background Reference 01

Keller D. M., Zeng X., et al., Mol. Cell 7:283-292 (2001) Keller D. M., Lu H. et al., J. Biol. Chem. 277:50206-50213 (2002) Trembley J. H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457 (2005) Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684 (2000)