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MEK5 (phospho Ser311 / Thr315) Antibody

Product Specifications

Background

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.

NCBI Gene ID

29568

Swiss Prot

Q62862

Accession Number

NP_001029159.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK5 (phospho-Ser311/ Thr315) antibody was raised against a peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S (p) I-A-K-T (p) -Y-V) derived from Rat MEK5.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-516

Calculated Molecular Weight

46 kDa

Specificity

This antibody detects endogenous level of MEK5 only when phosphorylated at serine 311 or threonine 315.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

Map2k5

NCBI Official Name

Mitogen activated protein kinase kinase 5

NCBI Organism

Rattus

Background Reference 01

English J. M., Vanderbilt C. A., Xu S., Marcus S., Cobb M. H. J. Biol. Chem. 270:28897-28902 (1995)

Other Product Names

Mek5, CDC2, CDC28A, CDKN1, P34CDC2, Map2k5

Tested Applications

WB

Protein ID

77157802

Physical Properties

Liquid

Curated Selection

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