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Cdc25B (phospho Ser149) Antibody

Product Specifications

Background

Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.

NCBI Gene ID

12531

Swiss Prot

Q9DBN8

Accession Number

NP_001104545.1

Host

Rabbit

Reactivity

Mouse, Rat

Immunogen

Cdc25B (Phospho-Ser149) antibody was raised against a peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

62 kDa

Specificity

This antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

Cdc25b

NCBI Official Name

Cell division cycle 25B

NCBI Organism

Murine

Background Reference 01

Li G, et al. (2010) Retrovirology. 7:59. Wang Z, et al. (2010) BMC Cancer. 10:233. Lucci MA, et al. (2010) Cell Oncol. 32 (5-6) : 361-72.

Other Product Names

AI604853, CDC25B

Tested Applications

WB

Protein ID

161621261

Physical Properties

Liquid

Frequently Asked Questions

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