FAK (phospho Tyr576 / Tyr577) Antibody
Product Specifications
Background
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
NCBI Gene ID
5747
Swiss Prot
Q05397
Accession Number
NP_005598.3
Host
Rabbit
Reactivity
Human
Immunogen
FAK (phospho-Tyr576/Tyr577) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 576/tyrosine 577 (S-T-Y (p) -Y (p) -K-A) derived from Human FAK.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-511
Calculated Molecular Weight
125 kDa
Specificity
This antibody detects endogenous level of FAK only when phosphorylated at tyrosine 576/577.
Applications Notes
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Symbol
PTK2
NCBI Official Name
Protein tyrosine kinase 2
NCBI Organism
Homo sapiens
Background Reference 01
Parsons, J. T. et al. (2000) Oncogene 19, 5606-5613Schaller, M. D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688. Cobb, B. S. et al. (1994) Mol. Cell. Biol. 14, 147-155. Chen, H. C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
Other Product Names
FAK, FADK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, EIF4F, EIF4G, EIF4GI, FADK 1, PTK2
Tested Applications
WB, IF
Protein ID
27886593
Physical Properties
Liquid
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