Cyclin E1 (phospho Thr395) Antibody

Product Specifications

Background

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.

NCBI Gene ID

898

Swiss Prot

P24864

Accession Number

NP_001229.1

Host

Rabbit

Reactivity

Human

Immunogen

Cyclin E1 (phospho-Thr395) antibody was raised against a peptide sequence around phosphorylation site of threonine 395 (L-L-T (p) -P-P) derived from Human Cyclin E1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48 kDa

Specificity

This antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.

Applications Notes

Western Blot: 1:1000

Symbol

CCNE1

NCBI Official Name

Cyclin E1

NCBI Organism

Homo sapiens

Background Reference 01

Won K. A., Reed S. I. EMBO J. 15:4182-4193 (1996) Welcker M., Singer J., Loeb K. R., Grim J., Bloecher A., Mol. Cell 12:381-392 (2003)

Other Product Names

CCNE, SIGLEC2, CCNE, CCNE1

Tested Applications

Protein ID

17318559

Physical Properties

Liquid

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS7081774 Inquire

Recombinant Bovine Translocon-associated protein subunit beta (SSR2), partial

Ask

View Details

MR204643L4V 200 µL

Gltpd2 (NM_146020) Mouse Tagged ORF Clone Lentiviral Particle

Ask

View Details

131339-ML750 100 µL

PIK3C2G (Phosphatidylinositol-4-phosphate 3-kinase C2 Domain-containing Subunit gamma, Phosphoinositide 3-kinase-C2-gamma, PI3K-C2gamma, PI3K-C2-gamma, PIK3C2G, PtdIns-3-kinase C2 Subunit gamma, MGC163149) (MaxLight 750) discontinued

Ask

View Details

NBP3-20778UV 0.1 mL

Rabbit Monoclonal Calcitonin Antibody (CALCA/8639R) [DyLight 350]

Ask

View Details

NBP2-76652G 0.1 mL

Mouse Monoclonal Maltose Binding Protein Antibody (R3.2) [DyLight 488]

Ask

View Details

RR213510L3 10 µg

Mpst (NM_138843) Rat Tagged Lenti ORF Clone

Ask

View Details