PLC gamma1 (phospho Tyr771) Antibody

Product Specifications

Background

PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor) -activated tyrosine kinase.

NCBI Gene ID

5335

Swiss Prot

P19174

Accession Number

NP_002651.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PLC-gamma1 (phospho-Tyr771) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y (p) -G-A) derived from Human PLC-γ1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

155 kDa

Specificity

This antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

PLCG1

NCBI Official Name

Phospholipase C, gamma 1

NCBI Organism

Homo sapiens

Background Reference 01

Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.