Smad1 (phospho Ser465) Antibody

Product Specifications

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) .

NCBI Gene ID

4086

Swiss Prot

Q15797

Accession Number

NP_001003688.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Smad1 (Phospho-Ser465) antibody was raised against a peptide sequence around phosphorylation site of serine 465 (I-S-S-V-S (p) ) derived from Human Smad1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

55 kDa

Specificity

This antibody detects endogenous level of Smad1 only when phosphorylated at serine 465.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SMAD1

NCBI Official Name

SMAD family member 1

NCBI Organism

Homo sapiens

Background Reference 01

Hiroaki Seto, et al. (2004) J Clin Invest. March 1; 113 (5) : 718–726Linyu Li, et al. (2004) Mol Cell Biol. January; 24 (2) : 856–864. Claire Nourry, et al. (2004) BMC Cell Biol. 5: 20