MEK1 (phospho Thr291) Antibody

Product Specifications

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

NCBI Gene ID

5604

Swiss Prot

Q02750

Accession Number

NP_002746.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK1 (Phospho-Thr291) antibody was raised against a peptide sequence around phosphorylation site of threonine 291 (P-R-T (p) -P-G derived from Human MEK1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Specificity

This antibody detects endogenous level of MEK1 only when phosphorylated at threonine 291.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MAP2K1

NCBI Official Name

Mitogen-activated protein kinase kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Kevin D. Burroughs, et, al. (2003) Mol. Cancer Res ; 1: 312. Michael J. Piatelli, et, al. (2002) J. Biol. Chem ; 277: 12144 - 12150. Margaret M. Morgan, et, al. (2001) J. Immunol ; 167: 5708. Herbert Schramek, et, al. (2003) Am J Physiol Cell Physiol, ; 285: C652 - C661.