CaMKII (phospho Thr286) Antibody

Product Specifications

Background

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity .

NCBI Gene ID

12322

Swiss Prot

P11798

Accession Number

NP_001273738.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CaMKII (Phospho-Thr286) antibody was raised against a peptide sequence around phosphorylation site of threonine 286 (Q-E-T (p) -V-D) derived from Human CaMKII.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

50 kDa

Specificity

This antibody detects endogenous level of CaMKII only when phosphorylated at threonine 286.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

Camk2a

NCBI Official Name

Calcium/calmodulin-dependent protein kinase II alpha

NCBI Organism

Homo sapiens

Background Reference 01

Pak JH, et al. Proc Natl Acad Sci U S A. 2000 Oct 10; 97 (21) : 11232-11237 Hudmon A, et al. J Cell Biol. Author manuscript; available in PMC 2006 May 7Miller P, et al. PLoS Biol. 2005 Apr; 3 (4) : e107Runyan JD, et al. Learn Mem. 2005 Mar; 12 (2) : 103-110.