A Synuclein (phospho Tyr136) Antibody

Product Specifications

Background

May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies) . Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

NCBI Gene ID

6622

Swiss Prot

P37840

Accession Number

NP_000336.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A-Synuclein (Phospho-Tyr136) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 136 (Q-D-Y (p) -E-P) derived from Human α -Synuclein.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

18 kDa

Specificity

This antibody detects endogenous level ofα -Synuclein only when phosphorylated at tyrosine 136.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

SNCA

NCBI Official Name

Synuclein, alpha (non A4 component of amyloid precursor)

NCBI Organism

Homo sapiens

Background Reference 01

Takahashi T, et al. J Biol Chem 2003 Oct 24; 278 (43) : 42225-33Ahn BH, et al. J Biol Chem 2002 Apr 05; 277 (14) : 12334-42Negro A, et al. FASEB J 2002 Feb; 16 (2) : 210-2Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.