Integrin beta3 (phospho Tyr785) Antibody

Product Specifications

Background

Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

NCBI Gene ID

3690

Swiss Prot

P05106

Accession Number

NP_000203.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Integrin beta3 (Phospho-Tyr785) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 785 (I-T-Y (p) -R-G) derived from Human Integrin β3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Specificity

This antibody detects endogenous level of Integrin β3 only when phosphorylated at tyrosine 785.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

ITGB3

NCBI Official Name

Integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)

NCBI Organism

Homo sapiens

Background Reference 01

Sujoy Bhattacharya, et al. (2006) Biochem J. August 1; 397 (Pt 3) : 437–447Courter DL, et al. (2005) J Biol Chem Apr 01; 280 (13) : 12145-12151Chandhoke SK, et al. (2004) J Cell Sci Mar 15; 117 (Pt 8) : 1431-1441