Cortactin (phospho Tyr466) Antibody

Product Specifications

Background

Cortactin is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.

NCBI Gene ID

2017

Swiss Prot

Q14247

Accession Number

NP_005222.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Cortactin (Phospho-Tyr466) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y (p) -E-T) derived from Human CORTACTIN.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

85 kDa

Specificity

This antibody detects endogenous level of Cortactin only when phosphorylated at tyrosine 466.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CTTN

NCBI Official Name

Cortactin

NCBI Organism

Homo sapiens

Background Reference 01

Luo C, et al. (2006) J Biol Chem ; 281 (40) : 30081-30093Head JA, et al. (2003) Mol Biol Cell ;14 (8) : 3216-3229Li Y, et al. (2000) J Biol Chem ; 275 (47) : 37187-37193Kinley AW, et al. (2003) Curr Biol ; 13 (5) : 384-393