Histone H2A.X (phospho Ser139) Antibody

Product Specifications

Background

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

NCBI Gene ID

3014

Swiss Prot

P16104

Accession Number

NP_002096.1

Host

Rabbit

Reactivity

Human

Immunogen

Histone H2A.X (Phospho-Ser139) antibody was raised against a peptide sequence around phosphorylation site of serine 139 (Q-A-S (p) -Q-E) derived from Human Histone H2A.X.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

15 kDa

Specificity

This antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

H2AFX

NCBI Official Name

H2A histone family, member X

NCBI Organism

Homo sapiens

Background Reference 01

Yaneva M, et al. (2005) Nucleic Acids Res. 33 (16) : 5320-5330. Tsukuda T, et al. (2006) Nature. Author manuscript; available in PMC 2006 March 6.

Other Product Names

H2AX, H2A.X, H2A/X, HNF3B, TCF3B, H2AFX, H2a/x, HIST5-2AX

Tested Applications

WB, IF

Protein ID

4504253

Physical Properties

Liquid

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