CDC2 (phospho Tyr15) Antibody

Product Specifications

Background

Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.

NCBI Gene ID

983

Swiss Prot

P06493

Accession Number

NP_001163877.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CDC2 (Phospho-Tyr15) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 15 (G-T-Y (p) -G-V) derived from Human CDC2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

34 kDa

Specificity

This antibody detects endogenous level of CDC2 only when phosphorylated at tyrosine 15.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

CDK1

NCBI Official Name

Cyclin-dependent kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Y Gu, et al. (1992) EMBO J. 11 (11) : 3995–4005. P Jin, et al. (1996) Cell Biology, Vol 134: 963-970, Tyagi, et al. (2005) Alpna1Carcinogenesis, Volume 26: 1978-1987 (10) Xiang S. et al. (1997) The EMBO Journal 16: 182–192