SP1 (phospho Thr739) Antibody

Product Specifications

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation) . Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

NCBI Gene ID

6667

Swiss Prot

P08047

Accession Number

NP_612482.2

Host

Rabbit

Reactivity

Human, Rat

Immunogen

SP1 (Phospho-Thr739) antibody was raised against a peptide sequence around phosphorylation site of threonine 739 (T-A-T (p) -P-S) derived from human SP1 .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

90 kDa

Specificity

This antibody detects endogenous levels of SP1 only when phosphorylated at threonine 739.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

SP1

NCBI Official Name

Sp1 transcription factor

NCBI Organism

Homo sapiens

Background Reference 01

Thiesen HJ, et al. (1990) Nucleic Acids Res. 18 (11) : 3203-9. Zutter MM, et al. (1997) Blood. 90 (2) : 678-89. Lou Z, et al. (2005) Cancer Res. 65 (3) : 1007-17