C Kit (phospho Tyr721) Antibody

Product Specifications

Background

This is the receptor for stem cell factor (mast cell growth factor) . It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K)

NCBI Gene ID

3815

Swiss Prot

P10721

Accession Number

NP_000213.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

C-Kit (Phospho-Tyr721) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 721 (N-E-Y (p) -M-D) derived from Human c-Kit.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

145 kDa

Specificity

This antibody detects endogenous level of c-Kit only when phosphorylated at tyrosine 721.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

KIT

NCBI Official Name

V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

NCBI Organism

Homo sapiens

Background Reference 01

Nocka, K. et al. (1990) EMBO J. 9, 1805-1813. Hirota, S. et al. (1998) Science 279, 577-580. Blume-Jensen, P. et al. (2000) Nat. Genet. 24, 157-162. Sattler, M. et al. (1997) J. Biol. Chem. 272, 10248-10253. Gommerman, J. L. et al. (1997) J. Biol. Chem. 272, 30519-30525.