Met (phospho Tyr1349) Antibody

Product Specifications

Background

Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.

NCBI Gene ID

4233

Swiss Prot

P08581

Accession Number

NP_000236.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Met (Phospho-Tyr1349) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1349 (E-H-Y (p) -V-H) derived from Human Met.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

156 kDa

Specificity

This antibody detects endogenous level of Met only when phosphorylated at tyrosine 1349.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

MET

NCBI Official Name

Met proto-oncogene

NCBI Organism

Homo sapiens

Background Reference 01

Fan S, et al. (2001) Mol Cell Biol; 21 (15) : 4968-4984Schiering N, et al. (2003) Proc Natl Acad Sci U S A; 100 (22) : 12654-12659Plopper GE, et al. (1995) Mol Biol Cell; 6 (10) : 1349-1365Ponzetto C, et al. (1993) Mol Cell Biol; 13 (8) : 4600-4608Jackson PA, et al. (2001) Plant Physiol; 127 (3) : 1065-1076