S6 Ribosomal Protein (phospho Ser235) Antibody

Product Specifications

Background

May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.

NCBI Gene ID

6194

Swiss Prot

P62753

Accession Number

NP_001001.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

S6 Ribosomal Protein (Phospho-Ser235) antibody was raised against a peptide sequence around phosphorylation site of serine 235 (R-L-S (p) -S-L) derived from Human S6 Ribosomal Protein.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

32 kDa

Specificity

This antibody detects endogenous level of S6 Ribosomal protein only when phosphorylated at serine 235.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

RPS6

NCBI Official Name

Ribosomal protein S6

NCBI Organism

Homo sapiens

Background Reference 01

McBride K, et al. (1998) Mol Cell Biol 18 (9) : 5073-5081. Williams AJ, et al. (2003) Plant Physiol 132 (4) : 2086-2097. Wilson MA, et al. (1997) Biochem J 325 (Pt 1) : 217-222. Arnesen T, et al. (2005) Biochem J 386 (Pt 3) : 433-443.