EGFR (phospho Tyr869) Antibody

Product Specifications

Background

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

NCBI Gene ID

1956

Swiss Prot

P00533

Accession Number

NP_005219.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

EGFR (Phospho-Tyr869) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 869 (K-E-Y (p) -H-A) derived from Human EGFR.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

175 kDa

Specificity

This antibody detects endogenous level of EGFR only when phosphorylated at tyrosine 869.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

EGFR

NCBI Official Name

Epidermal growth factor receptor

NCBI Organism

Homo sapiens

Background Reference 01

Inoue A, et al. (2005) PLoS Med; 2 (1) : e13Sun H, et al. (2004) EMBO J; 23 (1) : 100-110Kanner SB, et al. (1991) Mol Cell Biol; 11 (2) : 713-720Wu TT, et al. (1998) Mol Biol Cell; 9 (7) : 1661-1674O’Rourke DM, et al. (1997) Proc Natl Acad Sci U S A; 94 (7) : 3250-3255Goldstein DJ, et al. (1994) J Virol; 68 (7) : 4432-4441