FAK (phospho Tyr397) Antibody

Product Specifications

Background

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

NCBI Gene ID

5747

Swiss Prot

Q05397

Accession Number

NP_001186578.1

Host

Rabbit

Reactivity

Human, Rat

Immunogen

FAK (Phospho-Tyr397) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 397 (D-D-Y (p) -A-E) derived from Human FAK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

125 kDa

Specificity

This antibody detects endogenous level of FAK only when phosphorylated at Tyrosine 397.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PTK2

NCBI Official Name

Protein tyrosine kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Matsuya M. et. al. (1998) J. Biol. Chem. 273:1003-1014Fujita H. et. al. (1998) J. Biol. Chem. 273:26516-26521Le Romancer M. et. al. (2008) Mol. Cell 31:212-221