Tyrosine Hydroxylase (phospho Ser40) Antibody

Product Specifications

Background

The protein encoded by Tyrosine Hydroxylase is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

NCBI Gene ID

7054

Swiss Prot

P07101

Accession Number

NP_000351.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Tyrosine Hydroxylase (Phospho-Ser40) antibody was raised against a peptide sequence around phosphorylation site of serine 40 (R-Q-S (p) -L-I) derived from Human Tyrosine Hydroxylase (TH) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

55 - 60 kDa

Specificity

This antibody detects endogenous level of TyrosineHydroxylase only when phosphorylated at serine 40.

Applications Notes

Immunofluorescence: 1:100~1:200

Symbol

NCBI Official Name

Tyrosine hydroxylase

NCBI Organism

Homo sapiens

Background Reference 01

Vazin T, et al. Stem Cells. 2008 Jun;26 (6) : 1517-25Pistocchi A, et al. BMC Dev Biol. 2008 Mar 10;8:27 Fukakusa A, et al. J Pharmacol Sci. 2008 Feb;106 (2) : 321-4.