Raf1 (phospho Ser338) Antibody

Product Specifications

Background

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.

NCBI Gene ID

5894

Swiss Prot

P04049

Accession Number

NP_002871.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Raf1 (Phospho-Ser338) antibody was raised against a peptide sequence around phosphorylation site of serine 338 (R-D-S (p) -S-Y) derived from Human RAF.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

73 kDa

Specificity

This antibody detects endogenous level of Raf1 only when phosphorylated at serine 338.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

RAF1

NCBI Official Name

V-raf-1 murine leukemia viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Adams DG, et al. (2005) J Biol Chem. 280 (52) : 42644-54. Miura S, et al. (2003) Biochem Biophys Res Commun. 306 (4) : 924-9. Noser JA, et al. (2007) Mol Ther. 15 (8) : 1531-6.