Paxillin (phospho Tyr31) Antibody

Product Specifications

Background

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1) . This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

NCBI Gene ID

5829

Swiss Prot

P49023

Accession Number

NP_001074324.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Paxillin (Phospho-Tyr31) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Specificity

This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

PXN

NCBI Official Name

Paxillin

NCBI Organism

Homo sapiens

Background Reference 01

Davidson D, et al. (2001) EMBO J 20 (13) : 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3) : 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4) : 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3) : 929-939.