HSP90B (phospho Ser254) Antibody

Product Specifications

Background

Molecular chaperone. Has ATPase activity.

NCBI Gene ID

3326

Swiss Prot

P08238

Accession Number

NP_031381.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HSP90B (Phospho-Ser254) antibody was raised against a peptide sequence around phosphorylation site of serine 254 (V-G-S (p) -D-E) derived from Human HSP90B.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

92 kDa

Specificity

This antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

HSP90AB1

NCBI Official Name

Heat shock protein 90kDa alpha (cytosolic), class B member 1

NCBI Organism

Homo sapiens

Background Reference 01

Zhao R, Houry WA. (2007) Adv Exp Med Biol; 594: 27-36. Olsen JV, et al. (2006) Cell; 127 (3) : 635-648Ogiso H, et al. (2004) Biochemistry; 43 (49) : 15510-15519