APP (phospho 668) Antibody

Product Specifications

Background

APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy) . Multiple transcript variants encoding several different isoforms have been found for this gene.

NCBI Gene ID

351

Swiss Prot

P05067

Accession Number

NP_000475.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

APP (Phospho-668) antibody was raised against a peptide sequence around phosphorylation site of threonine 668 (A-V-T (p) -P-E) derived from Human APP.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

100 - 140 kDa

Specificity

This antibody detects endogenous level of APP only when phosphorylated at threonine 668.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

APP

NCBI Official Name

Amyloid beta (A4) precursor protein

NCBI Organism

Homo sapiens

Background Reference 01

Hung, A. Y. and Selkoe, D. J. (1994) EMBO J. 13, 534-542. Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427. Iijima, K. I. et al. (2000) J. Neurochem. 75, 1085-1091