Ephrin B2 (phospho Tyr330) Antibody

Product Specifications

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma) . Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca (2+) /calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

NCBI Gene ID

1948

Swiss Prot

P52799

Accession Number

NP_004084.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Ephrin-B2 (Phospho-Tyr330) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 330 (N-I-Y (p) -Y-K) derived from Human Ephrin-B2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

37 kDa

Specificity

This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 330.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

EFNB2

NCBI Official Name

Ephrin-B2

NCBI Organism

Homo sapiens

Background Reference 01

Chrencik JE, et al. (2006) J Biol Chem;281 (38) : 28185-28192. Kertesz N, et al. (2006) Blood; 107 (6) : 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA; 101 (15) : 5583-5588.