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Ephrin B2 (phospho Tyr316) Antibody

Product Specifications

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

NCBI Gene ID

1948

Swiss Prot

P52799

Accession Number

NP_004084.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Ephrin-B2 (Phospho-Tyr316) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 316 (P-V-Y (p) -I-V) derived from Human Ephrin-B2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-465

Calculated Molecular Weight

37 kDa

Specificity

This antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316.

Applications Notes

Western Blot: 1:500~1:1000

Symbol

EFNB2

NCBI Official Name

Ephrin-B2

NCBI Organism

Homo sapiens

Background Reference 01

Chrencik JE, et al. (2006) J Biol Chem;281 (38) : 28185-28192. Kertesz N, et al. (2006) Blood;107 (6) : 2330-2338. Noren NK, et al. (2004) Proc Natl Acad Sci USA;101 (15) : 5583-5588

Other Product Names

HTKL, EPLG5, Htk-L, LERK5, MEK1, PRKMK1, HTKL

Tested Applications

WB

Protein ID

4758250

Physical Properties

Liquid

Curated Selection

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