PLCgamma2 (phospho Tyr753) Antibody

Product Specifications

Background

The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

NCBI Gene ID

5336

Swiss Prot

P16885

Accession Number

NP_002652.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PLCgamma2 (Phospho-Tyr753) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y (p) -D-V) derived from Human PLCγ2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

150 kDa

Specificity

This antibody detects endogenous level of PLCγ2 only when phosphorylated at tyrosine 753.

Applications Notes

Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200

Symbol

PLCG2

NCBI Official Name

Phospholipase C, gamma 2 (phosphatidylinositol-specific)

NCBI Organism

Homo sapiens

Background Reference 01

Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029Rodriguez R, et al. (2003) Biochem J 374 : 269-280