CD357 Antibody [DTA-1] (APC)
Product Specifications
Background
The DTA-1 monoclonal antibody specifically reacts with mouse Glucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.
NCBI Gene ID
21936
Swiss Prot
O35714
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Clone
DTA-1
Conjugation
APC
Type
Primary Antibodies
Field of Research
Immunology
Purification
The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.
Concentration
Batch dependent
Buffer
Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.
Fragment
Rat IgG2b
Specificity
The DTA-1 monoclonal antibody specifically reacts with mouse Glucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.
Symbol
Tnfrsf18
NCBI Official Name
Tumor necrosis factor receptor superfamily, member 18
NCBI Organism
Mus musculus
Background Reference 01
Kataoka, H., Takakura, N., Nishikawa, S., Tsuchida, K., Kodama, H., Kunisada, T., ... Nishikawa, S. I. (1997) . Expressions of PDGF receptor alpha, c‐Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells.Development, growth differentiation,39 (6), 729-740.
Background Reference 02
Ishitobi, H., Matsumoto, K., Azami, T., Itoh, F., Itoh, S., Takahashi, S., Ema, M. (2010) . Flk1-GFP BAC Tg mice: an animal model for the study of blood vessel development.Experimental animals,59 (5), 615-622.
Background Reference 03
Yamashita, J., Itoh, H., Hirashima, M., Ogawa, M., Nishikawa, S., Yurugi, T., ... Nishikawa, S. I. (2000) . Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors.Nature,408 (6808), 92-96.
Other Product Names
AITR, Gitr, Tnfrsf18
Tested Applications
Flow
Physical Properties
Liquid
Curated Selection
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