CD357 Antibody [DTA-1] (PE)

Product Specifications

Background

The DTA-1 monoclonal antibody specifically reacts with mouse Glucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.

NCBI Gene ID

21936

Swiss Prot

O35714

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

DTA-1

Conjugation

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b

Specificity

Symbol

Tnfrsf18

NCBI Official Name

Tumor necrosis factor receptor superfamily, member 18

NCBI Organism

Mus musculus

Background Reference 01

Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... Sakaguchi, S. (2005) . Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells.The Journal of experimental medicine,202 (7), 885-891.

Background Reference 02

Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., Sakaguchi, S. (2002) . Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance.Nature immunology,3 (2), 135-142.

Background Reference 03

Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., Waldmann, H. (2003) . Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.Proceedings of the National Academy of Sciences,100 (25), 15059-15064.