CD357 Antibody [DTA-1]
Product Specifications
Background
The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone. GITR is also expressed in naïve mice by CD25+/CD4+/CD8a- thymocytes and on CD25+/CD4+/CD45RB-low splenocytes. Low levels were detected in splenic CD25+/CD4+/CD45RB-low T cells, B cells, dendritic cells and macrophages. A GITR ligand was detected on dendritic cells, macrophages and B cells.The DTA-1 antibody stimulates GITR and abrogates suppression by T regulatory cells (Treg), without affecting their proliferation. DTA-1 administration or the removal of GITR-expressing cells led to organ specific autoimmune disease.
NCBI Gene ID
21936
Swiss Prot
O35714
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Clone
DTA-1
Conjugation
Unconjugated
Type
Primary Antibodies, Functional Antibodies
Field of Research
Immunology
Purification
The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.
Concentration
Batch dependent
Buffer
Phosphate-buffered aqueous solution, ph7.2.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
The product should be stored undiluted at 4˚ C . Do not freeze.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=76-984
Fragment
Rat IgG2b
Specificity
The DTA-1 monoclonal antibody specifically reacts with MouseGlucocorticoid-Induced TNFR-related protein, also known as GITR and TNFRSF18, a 66-70 kDa homodimer glycoprotein, detected in the T cells treated with glucocorticoid dexamethasone.
Symbol
Tnfrsf18
NCBI Official Name
Tumor necrosis factor receptor superfamily, member 18
NCBI Organism
Mus musculus
Background Reference 01
Ko, K., Yamazaki, S., Nakamura, K., Nishioka, T., Hirota, K., Yamaguchi, T., ... Sakaguchi, S. (2005) . Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+ CD25+ CD4+ regulatory T cells.The Journal of experimental medicine,202 (7), 885-891.
Background Reference 02
Shimizu, J., Yamazaki, S., Takahashi, T., Ishida, Y., Sakaguchi, S. (2002) . Stimulation of CD25+ CD4+ regulatory T cells through GITR breaks immunological self-tolerance.Nature immunology,3 (2), 135-142.
Background Reference 03
Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., Waldmann, H. (2003) . Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells.Proceedings of the National Academy of Sciences,100 (25), 15059-15064.
Other Product Names
AITR, Gitr, Tnfrsf18
Tested Applications
Flow, Functional Assays, IHC, IF
Physical Properties
Liquid
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