CD279 Antibody [J43.1]

The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily. The PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-H2) are members of the B7 family. Pd-1 contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) and influences the peripheral tolerances and autoimmune diseases in mice. PD-1 is transiently expressed on CD4/CD8 thymocytes, it is upregulated in apoptotic cells, and it is expressed by activated myeloid and T and B cells. The binding of PD-1 to its ligands is blocked by the J43 antibody, which also enhances contact hypersensitivity and exacerbates acute Graft-versus-host disease, Experimental autoimmune encephalomyelitis and NOD diabetes. PD-1 seems to downregulate the immune response, as the development of splenomegaly and breakdown of peripheral tolerance in PD-1 deficient mice suggests.

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily.

Nishimura, H., Agata, Y., Kawasaki, A., Sato, M., Imamura, S., Minato, N., ... Honjo, T. (1996) . Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4CD8) thymocytes.International immunology,8 (5), 773-780.

Salama, A. D., Chitnis, T., Imitola, J., Ansari, M. J. I., Akiba, H., Tushima, F., ... Khoury, S. J. (2003) . Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis.The Journal of experimental medicine,198 (1), 71-78.

Carreno, B. M., Collins, M. (2002) . The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses.Annual review of immunology,20 (1), 29-53.