CD279 Antibody [J43.1]

Product Specifications

Background

The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily. The PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-H2) are members of the B7 family. Pd-1 contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) and influences the peripheral tolerances and autoimmune diseases in mice. PD-1 is transiently expressed on CD4/CD8 thymocytes, it is upregulated in apoptotic cells, and it is expressed by activated myeloid and T and B cells. The binding of PD-1 to its ligands is blocked by the J43 antibody, which also enhances contact hypersensitivity and exacerbates acute Graft-versus-host disease, Experimental autoimmune encephalomyelitis and NOD diabetes. PD-1 seems to downregulate the immune response, as the development of splenomegaly and breakdown of peripheral tolerance in PD-1 deficient mice suggests.

NCBI Gene ID

18566

Swiss Prot

Q02242

Host

Hamster

Reactivity

Mouse

Clonality

Monoclonal

Clone

J43.1

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology, Apoptosis

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Armenian Hamster IgG

Specificity

The J43.1 monoclonal antibody specifically reacts with mouse CD279, also known as PD-1 (programmed death-1), a 50-55 kDa glycoprotein of the Ig superfamily.

Symbol

Pdcd1

NCBI Official Name

Programmed cell death 1

NCBI Organism

Mus musculus

Background Reference 01

Nishimura, H., Agata, Y., Kawasaki, A., Sato, M., Imamura, S., Minato, N., ... Honjo, T. (1996) . Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4CD8) thymocytes.International immunology,8 (5), 773-780.

Background Reference 02

Salama, A. D., Chitnis, T., Imitola, J., Ansari, M. J. I., Akiba, H., Tushima, F., ... Khoury, S. J. (2003) . Critical role of the programmed death-1 (PD-1) pathway in regulation of experimental autoimmune encephalomyelitis.The Journal of experimental medicine,198 (1), 71-78.

Background Reference 03

Carreno, B. M., Collins, M. (2002) . The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses.Annual review of immunology,20 (1), 29-53.