CD284 Antibody [HTA125]

Product Specifications

Background

The HTA125 monoclonal antibody specifically reacts with human CD284, a 110kDA type I transmembrane signaling molecule known as the Toll-like Receptor 4 (TLR4) . CD284 is an important molecule in the innate immunity response to bacterial lipoproteins. It is expressed by monocytes, macrophages, and endothelial cells. The HTA125 antibody can block Lipopolysaccharide-induced cytokine production and immunoprecipitate human TLR4.

NCBI Gene ID

7099

Swiss Prot

O00206

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

HTA125

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Innate Immunity

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Mouse IgG2a, kappa

Specificity

The HTA125 monoclonal antibody specifically reacts with human CD284, a 110kDA type I transmembrane signaling molecule known as the Toll-like Receptor 4 (TLR4) .

Symbol

TLR4

NCBI Official Name

Toll-like receptor 4

NCBI Organism

Homo sapiens

Background Reference 01

Akashi, S., Ogata, H., Kirikae, F., Kirikae, T., Kawasaki, K., Nishijima, M., ... Miyake, K. (2000) . Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2.Biochemical and biophysical research communications,268 (1), 172-177.

Background Reference 02

Shimazu, R., Akashi, S., Ogata, H., Nagai, Y., Fukudome, K., Miyake, K., Kimoto, M. (1999) . MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.The Journal of experimental medicine,189 (11), 1777-1782.

Background Reference 03

Mirlashari, M. R., Lyberg, T. (2003) . Expression and involvement of Toll-like receptors (TLR) 2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis.Medical science monitor: international medical journal of experimental and clinical research,9 (8), BR316-24.