CD274 Antibody [10F.9G2] (PE)

Product Specifications

Background

The 10F.9G2 monoclonal antibody specifically reacts with mouse CD274, also known as B7-H1 or PD-L1, a 43 kDa glycoprotein of the B7 family of the immunoglobulin superfamily. CD274 is expressed on the B and T lymphocytes, natural killer cells, and dendritic cells. The receptor for the B7-H1 molecule is PD-1, which contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), and is expressed on activated B and T cells. The interaction between CD274 and PD-1 seems to downregulate the T and B immune responses.

NCBI Gene ID

29126

Swiss Prot

Q9NZQ7

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

10F.9G2

Conjugation

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2b, kappa

Specificity

The 10F.9G2 monoclonal antibody specifically reacts with mouse CD274, also known as B7-H1 or PD-L1, a 43 kDa glycoprotein of the B7 family of the immunoglobulin superfamily.

Symbol

CD274

NCBI Official Name

CD274 molecule

NCBI Organism

Homo sapiens

Background Reference 01

Akashi, S., Ogata, H., Kirikae, F., Kirikae, T., Kawasaki, K., Nishijima, M., ... Miyake, K. (2000) . Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2.Biochemical and biophysical research communications,268 (1), 172-177.

Background Reference 02

Shimazu, R., Akashi, S., Ogata, H., Nagai, Y., Fukudome, K., Miyake, K., Kimoto, M. (1999) . MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4.The Journal of experimental medicine,189 (11), 1777-1782.

Background Reference 03

Mirlashari, M. R., Lyberg, T. (2003) . Expression and involvement of Toll-like receptors (TLR) 2, TLR4, and CD14 in monocyte TNF-alpha production induced by lipopolysaccharides from Neisseria meningitidis.Medical science monitor: international medical journal of experimental and clinical research,9 (8), BR316-24.