CD274 Antibody [10F.9G2]

The 10F.9G2 monoclonal antibody specifically reacts with mouse CD274, also known as B7-H1 or PD-L1, a 43 kDa glycoprotein of the B7 family of the immunoglobulin superfamily. CD274 is expressed on the B and T lymphocytes, natural killer cells, and dendritic cells. The receptor for the B7-H1 molecule is PD-1, which contains an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), and is expressed on activated B and T cells. The interaction between CD274 and PD-1 seems to downregulate the T and B immune responses.

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

The 10F.9G2 monoclonal antibody specifically reacts with mouse CD274, also known as B7-H1 or PD-L1, a 43 kDa glycoprotein of the B7 family of the immunoglobulin superfamily.

Paterson, A. M., Brown, K. E., Keir, M. E., Vanguri, V. K., Riella, L. V., Chandraker, A., ... Sharpe, A. H. (2011) . The programmed death-1 ligand 1: B7-1 pathway restrains diabetogenic effector T cells in vivo.The Journal of Immunology,187 (3), 1097-1105

Koehn, B. H., Ford, M. L., Ferrer, I. R., Borom, K., Gangappa, S., Kirk, A. D., Larsen, C. P. (2008) . PD-1-dependent mechanisms maintain peripheral tolerance of donor-reactive CD8+ T cells to transplanted tissue.The Journal of Immunology,181 (8), 5313-5322.

Maier, H., Isogawa, M., Freeman, G. J., Chisari, F. V. (2007) . PD-1: PD-L1 interactions contribute to the functional suppression of virus-specific CD8+ T lymphocytes in the liver.The Journal of Immunology,178 (5), 2714-2720.