CD178 Antibody [NOK-1]

Product Specifications

Background

The NOK-1 monoclonal antibody specifically reacts with human CD178, which is the CD95 or Fas ligand. CD178 is a TNF superfamily type II transmembrane glycoprotein expressed by activated T and NK cells and is involved in Fas-mediated apoptosis of lymphocytes. CD178 is also expressed by monocytes, neutrophils, granulocytes and the parenchymal cells of the retina and cornea. The NOK-1 antibody has been reported to bind to COOH-terminus of the Fas ligand in the region associated with Fas binding.

NCBI Gene ID

356

Swiss Prot

P48023

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

NOK-1

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Apoptosis, Cancer

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The NOK-1 monoclonal antibody specifically reacts with human CD178, which is the CD95 or Fas ligand.

Symbol

FASLG

NCBI Official Name

Fas ligand (TNF superfamily, member 6)

NCBI Organism

Homo sapiens

Background Reference 01

Kayagaki, N., Kawasaki, A., Ebata, T., Ohmoto, H., Ikeda, S., Inoue, S., ... Yagita, H. (1995) . Metalloproteinase-mediated release of human Fas ligand.The Journal of experimental medicine,182 (6), 1777-1783.

Background Reference 02

Oyaizu, N., Adachi, Y., Hashimoto, F., McCloskey, T. W., Hosaka, N., Kayagaki, N., ... Pahwa, S. (1997) . Monocytes express Fas ligand upon CD4 cross-linking and induce CD4+ T cells apoptosis: a possible mechanism of bystander cell death in HIV infection.The Journal of Immunology,158 (5), 2456-2463.

Background Reference 03

Villunger, A., Egle, A., Marschitz, I., Kos, M., Bck, G., Ludwig, H., ... Greil, R. (1997) . Constitutive expression of Fas (Apo-1/CD95) ligand on multiple myeloma cells: a potential mechanism of tumor-induced suppression of immune surveillance.Blood,90 (1), 12-20.