CD127 Antibody [RDR5] (PE-Cy5)

Product Specifications

Background

The RDR5 monoclonal antibody specifically reacts with human CD127, a 60-90 kDA type I transmembrane glycoprotein also known as the IL-7 receptor alpha chain. CD127 binds with CD132 (gamma c) to forms the IL-7 receptor that is expressed on mature T cells and immature B cells. Since CD127 expression is downregulated in Treg cells, it can be used as a marker for differentiating them from common T cells. The binding of the RDR5 antibody can be blocked by pre-incubating the cells with human IL-7.

NCBI Gene ID

3575

Swiss Prot

P16871

Host

Mouse

Reactivity

Human

Clonality

Monoclonal

Clone

RDR5

Conjugation

PE-Cy5

Type

Primary Antibodies

Field of Research

Chemokines & Cytokines, Growth Factors

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The RDR5 monoclonal antibody specifically reacts with human CD127, a 60-90 kDA type I transmembrane glycoprotein also known as the IL-7 receptor alpha chain.

Symbol

IL7R

NCBI Official Name

Interleukin 7 receptor

NCBI Organism

Homo sapiens

Background Reference 01

Sallusto, F., Lenig, D., Frster, R., Lipp, M., Lanzavecchia, A. (1999) . Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.Nature,401 (6754), 708-712.

Background Reference 02

Yoshida, R., Nagira, M., Imai, T., Baba, M., Takagi, S., Tabira, Y., ... Yoshie, O. (1998) . EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC.International immunology,10 (7), 901-910.

Background Reference 03

Lee, N., Llano, M., Carretero, M., Ishitani, A., Navarro, F., Lpez-Botet, M., Geraghty, D. E. (1998) . HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.Proceedings of the National Academy of Sciences,95 (9), 5199-5204.