CD127 Antibody [A7R34] (Violet-450)

Product Specifications

Background

The A7R34 monoclonal antibody specifically reacts with the mouse CD127, the alpha subunit of the IL-7 receptor, expressed by immature B lymphocytes in the bone marrow, CD4-/CD8-, CD4+, and CD8+ thymocytes, and by mature T lymphocytes at low levels. Mature T cells express CD117 at low levels in the periphery.The A7R34 antibody prevents the interaction between IL-7 and its receptor and the binding of mAb SB/199, which also recognizes mouse CD127.BG Violet 450 conjugate is an alternative to the Pacific Blue, eFluor 450, or BD Horizon V450 dyes. It is excited by the violet (405 nm) laser, with a peak emission of 450nm.

NCBI Gene ID

16197

Swiss Prot

P16872

Host

Rat

Reactivity

Mouse

Clonality

Monoclonal

Clone

A7R34

Conjugation

Violet-450

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Rat IgG2a, kappa

Specificity

Symbol

Il7r

NCBI Official Name

Interleukin 7 receptor

NCBI Organism

Mus musculus

Background Reference 01

Sudo, T., Nishikawa, S. A. T. O. M. I., Ohno, N. O. R. I. K. O., Akiyama, N. A. O. K. O., Tamakoshi, M. A. S. A. T. A. D. A., Yoshida, H. I. S. A. H. I. R. O. (1993) . Expression and function of the interleukin 7 receptor in murine lymphocytes.Proceedings of the National Academy of Sciences,90 (19), 9125-9129.

Background Reference 02

Hashi, H., Yoshida, H., Honda, K., Fraser, S., Kubo, H., Awane, M., ... Nishikawa, S. I. (2001) . Compartmentalization of Peyers patch anlagen before lymphocyte entry.The Journal of Immunology,166 (6), 3702-3709.

Background Reference 03

Okuno, Y., Iwasaki, H., Huettner, C. S., Radomska, H. S., Gonzalez, D. A., Tenen, D. G., Akashi, K. (2002) . Differential regulation of the human and murine CD34 genes in hematopoietic stem cells.Proceedings of the National Academy of Sciences,99 (9), 6246-6251.