CD146 Antibody [P1H12] (PE)

Product Specifications

Background

The P1H12 monoclonal antibody reacts with CD146, 118kDA transmembrane glycoprotein that is also called the melanoma cell adhesion molecule (Mel-CAM), MUC18, Endo-CAM, and S-Endo. It is a prominently glycosylated protein with carbohydrates comprising the majority of its mass and it is expressed on melanoma, stromal, endothelial, and bone marrow fibroblasts. The P1H12 antibody cross reacts with human, mouse, canine, rabbit to the exclusion of rat protein.

NCBI Gene ID

84004

Swiss Prot

Q8R2Y2

Host

Mouse

Reactivity

Mouse

Clonality

Monoclonal

Clone

P1H12

Conjugation

Type

Primary Antibodies

Field of Research

Other

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Mouse IgG1, kappa

Specificity

The P1H12 monoclonal antibody reacts with CD146, 118kDA transmembrane glycoprotein that is also called the melanoma cell adhesion molecule (Mel-CAM), MUC18, Endo-CAM, and S-Endo.

Symbol

Mcam

NCBI Official Name

Melanoma cell adhesion molecule

NCBI Organism

Mus musculus

Background Reference 01

Chen, J. W., Cha, Y. I. N. G., Yuksel, K. U., Gracy, R. W., August, J. T. (1988) . Isolation and sequencing of a cDNA clone encoding lysosomal membrane glycoprotein mouse LAMP-1. Sequence similarity to proteins bearing onco-differentiation antigens.Journal of Biological Chemistry,263 (18), 8754-8758.

Background Reference 02

Grtzkau, A., Smorodchenko, A., Lippert, U., Kirchhof, L., Artuc, M., Henz, B. M. (2004) . LAMP‐1 and LAMP‐2, but not LAMP‐3, are reliable markers for activation‐induced secretion of human mast cells.Cytometry Part A,61 (1), 62-68.

Background Reference 03

Sarafian, V., Jadot, M., Foidart, J. M., Letesson, J. J., Van den Brule, F., Castronovo, V., ... Coninck, W. D. (1998) . Expression of Lamp‐1 and Lamp‐2 and their interactions with galectin‐3 in human tumor cells.International journal of cancer,75 (1), 105-111.