CD154 Antibody [MR1] (FITC)

Product Specifications

Background

The MR1 monoclonal antibody specifically reacts with mouse CD154, a 39kDA transmembrane glycoprotein also known as CD40 ligand and gp39. CD154 is expressed on activated T cells and plays a role in B-T cell costimulation. The interaction of CD154 with CD40 induces B cell cycle entry and the signaling for T-dependent B cell activation. By interfering with this interaction, the MR1 antibody is reported to inhibit T and B lymphocyte activation.

NCBI Gene ID

21947

Swiss Prot

P27548

Host

Hamster

Reactivity

Mouse

Clonality

Monoclonal

Clone

MR1

Conjugation

FITC

Type

Primary Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C and should be protected from prolonged exposure to light. Do not freeze.

Fragment

Armenian Hamster IgG

Specificity

The MR1 monoclonal antibody specifically reacts with mouse CD154, a 39kDA transmembrane glycoprotein also known as CD40 ligand and gp39.

Symbol

Cd40lg

NCBI Official Name

CD40 ligand

NCBI Organism

Mus musculus

Background Reference 01

Noelle, R. J., Roy, M., Shepherd, D. M., Stamenkovic, I., Ledbetter, J. A., Aruffo, A. (1992) . A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells.Proceedings of the National Academy of Sciences,89 (14), 6550-6554.

Background Reference 02

Roy, M., Aruffo, A., Ledbetter, J., Linsley, P., Kehry, M., Noelle, R. (1995) . Studies on the interdependence of gp39 and B7 expression and function during antigen‐specific immune responses.European journal of immunology,25 (2), 596-603.

Background Reference 03

Van den Eertwegh, A. J., Noelle, R. J., Roy, M. E. E. N. A. K. S. H. I., Shepherd, D. M., Aruffo, A., Ledbetter, J. A., ... Claassen, E. (1993) . In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. I. In vivo expression of CD40 ligand, cytokines, and antibody production delineates sites of cognate TB cell interactions.The Journal of experimental medicine,178 (5), 1555-1565.