CD28 Antibody [37.51]

Product Specifications

Background

The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer. It is expressed on most thymocytes, at low density on most of the CD4+ and CD8+ peripheral T lymphocytes, and on the natural killer cells. It is additionally found on activated splenocytes, mast cells, and thymocytes. CD 28 is a costimulator of T lymphocytes and a ligand for CD80 (B7-1), and CD86 (B7-2) . The CD28 signaling stimulates the expression of IL-2 and IL-2 receptor and the cytotoxicity of CD3-activated T lymphocytes.The 37.51 antibody stimulates the proliferation of T lymphocytes and natural killer cells and their respective cytokine production.

NCBI Gene ID

12487

Swiss Prot

P31041

Host

Hamster

Reactivity

Mouse

Clonality

Monoclonal

Clone

37.51

Conjugation

Unconjugated

Type

Primary Antibodies, Functional Antibodies

Field of Research

Immunology

Purification

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

Concentration

Batch dependent

Buffer

Phosphate-buffered aqueous solution, ph7.2.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

The product should be stored undiluted at 4˚ C . Do not freeze.

Fragment

Golden Syrian Hamster IgG

Specificity

The 37.51 monoclonal antibody specifically reacts with mouse CD28 molecule, a 45 kDa homodimer.

Symbol

Cd28

NCBI Official Name

CD28 antigen

NCBI Organism

Mus musculus

Background Reference 01

Nandi, D., Gross, J. A., Allison, J. P. (1994) . CD28-mediated costimulation is necessary for optimal proliferation of murine NK cells.The Journal of Immunology,152 (7), 3361-3369.

Background Reference 02

Gross, J. A., Callas, E. L. E. N. I., Allison, J. P. (1992) . Identification and distribution of the costimulatory receptor CD28 in the mouse.The Journal of Immunology,149 (2), 380-388.

Background Reference 03

Grossi, J. A., Raulet, D. H., Allison, J. P. (1992) . CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones.Nature,356, 607-9.

Background Reference 04

Nishio, M., Spielman, J., Lee, R. K., Nelson, D. L., Podack, E. R. (1996) . CD80 (B7. 1) and CD54 (intracellular adhesion molecule-1) induce target cell susceptibility to promiscuous cytotoxic T cell lysis.The Journal of Immunology,157 (10), 4347-4353.