CD28 Antibody [CD28.2]

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some subregions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

The monoclonal antibody was purified utilizing affinity chromatography. The endotoxin level is determined by LAL test to be less than 0.01 EU/µg of the protein.

The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes.

Nuns, J., Klasen, S., Ragueneau, M., Pavon, C., Couez, D., Mawas, C., ... Olive, D. (1993) . CD28 mAbs with distinct binding properties differ in their ability to induce T cell activation: analysis of early and late activation events.International immunology,5 (3), 311-315.

Karlsson, I., Malleret, B., Brochard, P., Delache, B., Calvo, J., Le Grand, R., Vaslin, B. (2007) . FoxP3+ CD25+ CD8+ T-cell induction during primary simian immunodeficiency virus infection in cynomolgus macaques correlates with low CD4+ T-cell activation and high viral load.Journal of virology,81 (24), 13444-13455.